Fiber-based microendoscopic multiphoton imaging
نویسندگان
چکیده
We developed a multiphoton microscope which integrates an all normal dispersion fiber laser, a double cladding photonic crystal fiber and a MEMS mirror scanner based hand-held probe. The fiber laser has a central wavelength of 1.06um,a repetition rate of 76MHz and maximum average output power of more than 1W. The MEMS mirror based probe is compact and Second harmonic generation and two photon excited fluorescence images of biological sample were demonstrated.
منابع مشابه
Beam-scanning Multiphoton Multimodality Endoscopy
Two-photon-exciter fluorescence (2PEF) imaging and second-harmonic generation (SHG) microscopy has become well-established techniques for biomedical imaging [1]. Integration of fibers into the multiphoton microscopy system further enables in vivo and noninvasive investigation of biological samples. Nonetheless the broadening of ultrashort pulses in fibers has greatly hampered the development of...
متن کاملFiber-based combined optical coherence and multiphoton endomicroscopy.
A fiber-based multimodal imaging system that combines multiphoton microscopy (MPM) with Fourier domain optical coherence microscopy (OCM) is reported. The system uses a fiber-based femtosecond laser, a fiber coupler, and a double-clad fiber (DCF) device. The fiber laser has a central wavelength of 1.04 μm and bandwidth of 29 nm. Longer excitation wavelength is used to increase penetration depth...
متن کاملMultiphoton endoscopy.
Despite widespread use of multiphoton fluorescence microscopy, development of endoscopes for nonlinear optical imaging has been stymied by the degradation of ultrashort excitation pulses that occurs within optical fiber as a result of the combined effects of group-velocity dispersion and self-phase modulation. We introduce microendoscopes (350-1000 microm in diameter) based on gradient-index mi...
متن کاملDeveloping compact multiphoton systems using femtosecond fiber lasers.
We implement a fiber-delivered compact femtosecond fiber laser at 1,030-nm wavelength in multiphoton imaging. The laser pulse duration is 150 fs, the average power is 200 mW, and the repetition rate is 40 MHz. The laser measures 200 x 160 x 45 mm in size and its output is delivered through a photonic bandgap fiber. Intrinsic second-harmonic generation signal is excited from rat tail tendon and ...
متن کاملDesign and implementation of fiber-based multiphoton endoscopy with microelectromechanical systems scanning.
A multiphoton endoscopy system has been developed using a two-axis microelectromechanical systems (MEMS) mirror and double-cladding photonic crystal fiber (DCPCF). The MEMS mirror has a 2-mm-diam, 20-deg optical scanning angle, and 1.26-kHz and 780-Hz resonance frequencies on the x and y axes. The maximum number of resolvable focal spots of the MEMS scanner is 720 x 720 on the x and y axes, whi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 2 شماره
صفحات -
تاریخ انتشار 2010